Wyo. Code R. 048-0010-3
Chemical Analysis of Blood Alcohol
Chapter 3: Procedures for Analysis
Effective Date: 02/11/1991 to 01/26/2004
Rule Type: Superceded Rules & Regulations
Reference Number: 048.0010.3.02111991
Section 1. Procedural controls.
(a) Analytical procedures for breath alcohol analysis should include the following controls in conjunction with the testing of each subject:
(i) Subject must be observed for a minimum of twenty (20) minutes prior to testing to prevent residual mouth alcohol. All foreign material (except dentures) must be removed from the mouth at the start of observation period and subject must not be allowed to smoke, eat, drink, etc. If the subject vomits or regurgitates, the observation period must be restarted after cleaning the mouth. A burp or belch will not affect the test; therefore, the observation time can continue.
(ii) A blank analysis with a result of less than 0.010%.
(iii) Analysis of a suitable reference sample the result of which analysis must agree within the limits of +5% to -10% of the expected value.
(iv) Duplicate breath specimens must be analyzed. If they do not agree within 0.020% a third specimen should be run. The lowest value of the pair(s) that agree within 0.020% will be reported. Agreement within 0.020% verifies the results and demonstrates that no significant errors, such as residual mouth alcohol, voltage changes, electromagnetic interference, nonalveolar breath samples, etc. have occurred. If a pair of results agreeing within 0.020% cannot be found among the three results, a blood or urine test should be taken or the breath test should be started over, using none of the previous three results.
(v) Analysis must be run using an approved checklist provided by the state agency.
(b) Analytical procedures for blood and urine alcohol analyses should include the following controls in conjunction with any sample or series of samples analyzed sequentially or simultaneously:
(i) A blank analysis where appropriate with a result of less than 0.005%.
(ii) Analysis of a suitable reference sample the result of which analysis must agree within the limits of +5% or -10% of the expected value.
(iii) Each sample shall be analyzed at least in duplicate, and the average shall be reported. The results must agree within 5% or within 0.005% if the alcohol concentration is less than 0.100%.
Section 2. Reporting of results.
(a) Results, except for proficiency test samples, shall be reported to two places, the third place being dropped (i.e., 0.148% shall be reported as 0.14%).
(b) Results of analyses shall be reported as percent (%) alcohol concentration using the definitions in Chapter 1, section 3(b).
Section 3. Blood Sample Collection Procedures.
(a) Use standard medical procedures and equipment for obtaining blood samples. (b) The collection site should be cleansed using a preparation that does not contain ethyl alcohol. (c) The collection tube shall contain an appropriate anticoagulant and a preservative, such as potassium oxalate or EDTA (ethylenediaminetetraacetic acid) and a minimum of 0.1% sodium fluoride. (d) Sample tubes and/or mailing containers shall bear tamperproof seals that will maintain the chain of evidence.
Section 4. Urine Sample Collection Procedures.
(a) Two (2) samples shall be collected approximately thirty (30) minutes apart and the reported value shall be based on the results of the second sample which will show the alcohol concentration at a point in time half-way between the two samples. The loss of the first sample shall not invalidate the results if the actual time of voiding the bladder was recorded. (b) The sample tube shall contain an appropriate preservative such as a minimum of 0.1% sodium fluoride. (c) Sample may be collected in a clean container and transferred to a sample tube. (d) Sample tubes and/or mailing containers shall bear tamperproof labels that will maintain the chain of evidence.