Wyo. Code R. 048-0010-3
Chemical Analysis of Blood Alcohol
Chapter 3: Procedures for Analysis
Effective Date: 12/11/2013 to Current
Rule Type: Current Rules & Regulations
Reference Number: 048.0010.3.12112013
PROCEDURES FOR ANALYSIS
Section 1. Procedural controls.
(a) Analytical procedures for breath alcohol analysis should include the following controls in conjunction with the testing of each subject:
(i) Subject must be observed for a minimum of fifteen (15) minutes prior to testing to prevent residual mouth alcohol. All foreign material (except dentures) must be removed from the mouth at the start of observation period and subject must not be allowed to smoke, eat, drink or place anything in their mouth. If the subject vomits or regurgitates, the observation period must be restarted after rinsing the mouth with water. A dry burp or belch will not affect the test; therefore, the observation time can continue.
(ii) A blank analysis with a result less than 0.005%.
(iii) Analysis of a suitable reference sample whose result shall agree within the limits of +5% to -10% of the expected value.
(iv) Duplicate breath specimens must be analyzed. If the duplicate analyzed specimens do not agree within 0.020% of each other then a third specimen must be analyzed. The lowest value of the pair that agrees within 0.020% of each other shall be reported. Agreement within 0.020% verifies the results and demonstrates that no significant errors, such as residual mouth alcohol, voltage changes, electromagnetic interference, non-alveolar breath, etc. have occurred. If a pair of results agreeing within 0.020% cannot be found among the three results, a blood or urine test should be taken or the breath test started over, discarding all previous results.
(v) Analysis must be run using an approved checklist provided by the state agency.
(b) Analytical procedures for blood and urine alcohol analyses should include the following controls in conjunction with any sample or series of samples analyzed sequentially or simultaneously:
(i) A blank analysis with a result of less than 0.005%.
(ii) Analysis of one or more suitable reference samples and controls, the results of which must agree within the limits of +5% or -10% of the expected value.
(iii) Each subject sample shall be analyzed in duplicate and the lower of the two shall be reported. The results must agree within 5% or within 0.005% if the alcohol concentration is less than 0.100%.
Section 2. Reporting of results.
(a) The results will be reported in a manner consistent with the analytical capability of the instrumentation. That determination will be incorporated in the methods approved by the Department of Health.
(b) Results of analyses shall be reported as percent (%) alcohol concentration using the definitions in Chapter 1, section 3(b).
Section 3. Blood Sample Collection Procedures.
The blood samples will be:
(a) Collected using standard medical procedures and equipment for obtaining blood samples in the presence of the arresting officer or other responsible person who can authenticate the specimen(s).
(b) Collected using sterile equipment. The skin at the area of puncture should be thoroughly cleansed and disinfected with an aqueous solution of nonvolatile antiseptic. Alcohol solutions should not be used as a skin antiseptic.
(c) Collected utilizing tubes that contain an appropriate anticoagulant and a preservative, such as potassium oxalate or EDTA (ethylenediaminetetraacetic acid) and a minimum of 0.1% sodium fluoride.
(d) Collected using tubes and/or mailing containers bearing tamper evident seals that will maintain the chain of custody.
(e) Collected by venipuncture by a physician, nurse, paramedic, emergency medical technician, medical technologist or a person whose training and normal duties include withdrawing blood specimens under the supervision of a physician or nurse.
(f) Collected only in an appropriate clinical or public safety facility (e.g., hospital, medical clinic, ambulance, police station, fire station or approved facility). In no event will the collection of blood specimens interfere with the provision of essential medical care or the ready availability of emergency medical services to the public.
Section 4. Urine Sample Collection Procedures.
(a) Two (2) samples shall be collected approximately thirty (30) minutes apart and the reported value shall be based on the results of the second sample that will show the alcohol concentration at a point in time halfway between the two samples. The loss of the first sample shall not invalidate the results if the actual time of voiding the bladder was recorded.
(b) The collection tube shall contain a minimum of 0.1% sodium fluoride as a preservative.
(c) The sample may be collected in a clean container and transferred to the sample tube.
(d) Sample tubes and/or mailing containers shall bear tamper evident seals that will maintain the chain of custody.