Mo. Code Regs. Ann. tit. 19, § 25-30.080
Approval of Methods for the Analysis of Blood and Urine for the Presence of Drugs
Effective Oct 30, 2001sections 192.006, 306.114, 306.117, 577.020, 577.023, 577.026, 577.029, 577.031, 577.033, 577.037, 577.039 and 577.041, RSMo 2000.* This rule previously filed as 19 CSR 20-30.080. Original rule filed July 15, 1988, effective Sept. 29, 1988. Changed to 19 CSR 20- 30.080 Jan. 1, 1995. Emergency amendment filed May 10, 2001, effective May 22, 2001, expired Nov. 17, 2001. Amended: Filed May 10, 2001, effective Oct. 30, 2001. *Original authority: 192.006, RSMo 1993, amended 1995; 306.114, RSMo 1993; 306.117, RSMo 1993; 577.020, RSMo 1977, amended 1982, 1983, 1996, 1998; 577.023, RSMo 1982, amended 1983, 1991, 1993, 1998; 577.026, RSMo 1982; 577.029, RSMo 1982; 577.031, RSMo 1982; 577.033, RSMo 1982; 577.037, RSMo 1982, amended 1983, 1988, 1993, 1996; 577.039, RSMo 1982, amended 1996; and 577.041, RSMo 1982, amended 1987, 1991, 1993, 1996, 1998Missouri State Public Health Laboratory
PURPOSE: This rule establishes the approved methods for the analysis of blood and urine for the presence of drugs.
(1) Samples of blood or urine shall be collected in accordance with the provision of sections 577.029, and 306.111–306.119, RSMo and a sufficient volume of sample shall be collected to provide for duplicate testing.
- (A) Blood samples shall be collected in a clean, dry container that has an air-tight, inert stopper. If whole blood or plasma is required, an anticoagulant may be used that is appropriate for the test method.
- (B) Urine specimens shall be collected in clean, dry containers. Preservatives may be used that are appropriate for the test method. Specimens shall be refrigerated if not tested within one (1) day of collection.
- (2) An individual shall have a valid Type I permit in order to perform analyses of blood and urine for the presence of drugs.
- (3) The laboratory in which these analyses are performed shall have a director who shall assume full responsibility for the accuracy of tests and reports.
- (4) The laboratory in which these analyses are performed shall participate in a proficiency testing program that provides at least three
- (3) sets of samples per calendar year and covers the screening and confirmatory methods that are used.
(5) The following methodologies are approved for the analysis of blood and urine for the presence of drugs:
- (A) Enzyme immunoassay (EIA);
- (B) Fluorescence immunoassay (FIA);
- (C) Radioimmunoassay (RIA); 19 CSR 25-30
- (D) Gas-liquid chromatography (GLC);
- (E) Thin layer chromatography (TLC);
- (F) High-pressure liquid chromatography (HPLC);
- (G) Ultra-violet spectrophotometry (UV); and
- (H) Gas chromatography/mass spectrometry (GC/MS).
- (6) All positive results found upon initial testing shall be confirmed by GC/MS. TLC and HPLC methods may be used in conjunction with GC/MS for confirmation.
AUTHORITY: sections 192.006, 306.114, 306.117, 577.020, 577.023, 577.026, 577.029, 577.031, 577.033, 577.037, 577.039 and 577.041, RSMo 2000.* This rule previously filed as 19 CSR 20-30.080. Original rule filed July 15, 1988, effective Sept. 29, 1988. Changed to 19 CSR 20- 30.080 Jan. 1, 1995. Emergency amendment filed May 10, 2001, effective May 22, 2001, expired Nov. 17, 2001. Amended: Filed May 10, 2001, effective Oct. 30, 2001. *Original authority: 192.006, RSMo 1993, amended 1995; 306.114, RSMo 1993; 306.117, RSMo 1993; 577.020, RSMo 1977, amended 1982, 1983, 1996, 1998; 577.023, RSMo 1982, amended 1983, 1991, 1993, 1998; 577.026, RSMo 1982; 577.029, RSMo 1982; 577.031, RSMo 1982; 577.033, RSMo 1982; 577.037, RSMo 1982, amended 1983, 1988, 1993, 1996; 577.039, RSMo 1982, amended 1996; and 577.041, RSMo 1982, amended 1987, 1991, 1993, 1996, 1998.