Authority: IC 15-18-2

Affected: IC 15-18-2-14

Sec. 9. The following procedures must be used for testing milk:

1. (1) Heat samples to one hundred (100) degrees Fahrenheit in a water bath thermostatically controlled at one hundred five (105) degrees Fahrenheit. Mix by gently shaking the container or by pouring from one (1) container to another.
2. (2) Carefully measure exactly seventeen and six-tenths (17.6) milliliters of milk into an eight percent (8%) Babcock test bottle marked Standard Glassware Purdue (SGP).
3. (3) Cool to fifty-eight (58) to seventy (70) degrees Fahrenheit.
4. (4) Add seventeen and five-tenths (17.5) milliliters of sulfuric acid at a temperature of fifty-eight (58) to seventy (70) degrees Fahrenheit and specific gravity of one and eighty-two hundredths (1.82) to one and eighty-three hundredths (1.83).
5. (5) Immediately after adding acid to milk, shake for at least one (1) minute.
6. (6) Centrifuge for five (5) minutes in a centrifuge heated to one hundred twenty-six (126) degrees Fahrenheit.
7. (7) Add soft water at one hundred twenty-six (126) degrees Fahrenheit. Fill to the base of the bottle neck.
8. (8) Centrifuge for two (2) minutes in a centrifuge heated to one hundred twenty-six (126) degrees Fahrenheit.
9. (9) Add soft water at one hundred twenty-six (126) degrees Fahrenheit to bring the fat column to near the top of the graduated neck of the bottle.
10. (10) Centrifuge for one (1) minute in a centrifuge heated to one hundred twenty-six (126) degrees Fahrenheit.
11. (11) Place sample bottles in a water bath maintained at one hundred eighteen (118) degrees Fahrenheit for five (5) minutes with the water level above the top of the fat columns.
12. (12) Read to the nearest one-tenth percent (0.1%) immediately after removal from the water using a good source of indirect light.

(Creamery Examining Board; 365 IAC 2-1-9; filed Jun 20, 2001, 3:50 p.m.: 24 IR 3622; readopted filed Jul 15, 2008, 12:53 p.m.: 20080813-IR-365080121RFA; readopted filed Oct 23, 2014, 11:08 a.m.: 20141119-IR-365140351RFA; readopted filed Nov 23, 2020, 1:34 p.m.: 20201223-IR-365200088RFA; readopted filed February 23, 2026, 2:04 p.m.: 20260325-IR-365250384RFA)