Authority: IC 15-17-3-21

Affected: IC 15-17-3-13; IC 15-17-7

Sec. 7. (a) The state veterinarian shall quarantine all herds in which reactor cattle and bison are found. The state veterinarian shall conduct an epidemiological investigation whenever facts suggest tuberculosis may be present in animals in the state or from the state. Affected herds shall remain under quarantine until such time as the state veterinarian releases the quarantine.

(b) Feeder calves under twelve (12) months of age from quarantined herds may be moved intrastate to an approved feedlot if each animal to be moved has tested negative for tuberculosis using the CFT test within the sixty (60) days prior to movement.

(c) The state veterinarian may release a tuberculosis quarantine of a herd in which tuberculosis infection has been confirmed when the following conditions are met:

1. (1) All reactors have been condemned and removed from the herd.

2. (2) The remaining animals in the herd test negative for tuberculosis as follows:

   1. (A) A herd tuberculosis test followed by a second tuberculosis test conducted between sixty (60) and one hundred twenty (120) days after the first, and a third tuberculosis test is conducted between one hundred eighty (180) and two hundred forty (240) days after the first herd test.
   2. (B) An annual herd tuberculosis test is conducted in each of the three (3) years following the year in which the tests in clause (A) are conducted.
3. (3) If the herd is in an area where tuberculosis is known to affect one (1) or more species of wildlife, the herd owner shall develop a herd plan that will mitigate the exposure of the herd to tuberculosis and submit the plan to the state veterinarian for approval. The state veterinarian shall evaluate the herd plan and approve plans that will likely mitigate exposure to tuberculosis.
4. (4) The premises is cleaned and disinfected under subsection (g).

All animals moved from the premises prior to quarantine release shall be moved directly to slaughter under a permit issued by the state veterinarian.

(d) The state veterinarian may release a tuberculosis quarantine of a herd in which:

1. (1) no gross lesion (NGL) reactor or reactors occur;
2. (2) selected tissues are found negative on histopathology; and
3. (3) no evidence of Mycobacterium bovis infection has been disclosed;

after a negative retest of the entire herd is completed at least sixty (60) days after the slaughter of the NGL reactors. The state veterinarian may waive the retest requirement if advised to do so by the United States Department of Agriculture.

(e) The state veterinarian may release a tuberculosis quarantine of any herd if:

1. (1) the entire herd is depopulated and the premises is cleaned and disinfected under subsection (g); and
2. (2) any waiting period established under subsection (h) is satisfied.

(f) Herds in which at least one (1) suspect and no reactor animals are disclosed may be released after:

1. (1) all suspects are retested and classified negative or shipped directly to slaughter under supervision of the state veterinarian; and
2. (2) no evidence of tuberculosis infection is found in antemortem testing and examination and the epidemiological investigation.

(g) Premises where reactor cattle or bison have been maintained shall be thoroughly cleaned and disinfected with a disinfectant approved by the state veterinarian and in a manner approved by the state veterinarian. Cleaning and disinfection must be completed within fifteen (15) days after the last reactor is removed from the premises unless the state veterinarian grants an extension of time. The state veterinarian may grant an extension of time for cleaning and disinfecting if an extension will not impair effective disease control.

(h) The state veterinarian may order a premises or a portion of the premises remain vacant for up to sixty (60) days after cleaning and disinfecting is completed before any animals are moved to the premises or portion of a premises if necessary to ensure tuberculosis control and eradication.

(i) Herds containing suspect cattle and bison shall be quarantined until all suspect animals are tested and classified negative for tuberculosis or are shipped directly to a slaughtering establishment under permit issued by the state veterinarian. Suspect animals shall be evaluated as follows:

1. (1) Suspects in herds containing only animals that are suspects on the CFT test may be released from quarantine when the suspect animals:

   1. (A) test negative for tuberculosis using the CCT test within ten (10) days of the caudal fold injection;
   2. (B) are retested and found to be negative for tuberculosis using the CCT test sixty (60) or more days after the caudal fold test injection;
   3. (C) are retested negative for tuberculosis on the bovine interferon gamma assay tuberculin test within thirty (30) days of the caudal fold injection if the state veterinarian has approved the use of the bovine interferon gamma assay test; or
   4. (D) are shipped to a slaughtering establishment under permit issued by the state veterinarian and examined for tuberculosis postmortem.

2. (2) An animal that is suspect for tuberculosis using the CCT test meets one (1) of the following sets of criteria:

   1. (A) The animal is retested using the CCT test more than sixty (60) days after the prior comparative cervical injection.
   2. (B) The animal is shipped directly to a slaughtering establishment under permit issued by the state veterinarian.
3. (3) The state veterinarian may classify an animal a reactor if the animal tests in the suspect zone on two (2) successive CCT tests or positive on two (2) successive bovine interferon gamma assay tests.

4. (4) Animals positive on the bovine interferon gamma assay tuberculin test and classified as suspects must meet one (1) of the following sets of criteria:

   1. (A) The animal is retested negative on the bovine interferon gamma assay test within thirty (30) days of the CFT test injection with approval of the state veterinarian.
   2. (B) The animal is shipped directly to a slaughtering establishment under permit issued by the state veterinarian.

(j) Postmortem examinations of animals under this article shall be witnessed by a state or federal official and selected tissue specimens, including any tissue with granulomatous appearing lesions and representative head and thoracic lymph nodes, will be submitted for laboratory examination.

(k) The state veterinarian may order the following animals tested for tuberculosis:

1. (1) All cattle and bison in a herd from which a reactor originated and all animals that are known to have associated with reactor cattle or bison.
2. (2) Livestock herds within a ten (10) mile radius of the location of livestock or free ranging wildlife that is diagnosed with bovine tuberculosis.
3. (3) Animals in herds that are adjacent to the location of suspect or exposed animals.
4. (4) Animals in herds that received or otherwise had contact with reactor, suspect, or exposed animals.

Nothing in this subsection is intended to limit the authority of the state veterinarian to order tuberculosis tests under any other section of this article or any other law.

(Indiana State Board of Animal Health; 345 IAC 2.5-3-7; filed Apr 13, 2005, 12:30 p.m.: 28 IR 2683; readopted filed Nov 1, 2011, 3:50 p.m.: 20111130-IR-345110312RFA; readopted filed Jul 17, 2017, 9:06 a.m.: 20170816-IR-345170165RFA; readopted filed Oct 4, 2023, 9:15 a.m.: 20231101-IR-345230625RFA; readopted filed Oct 15, 2025, 3:08 p.m.: 20251105-IR-345240625RFA)