Fla. Admin. Code R. 62-610.652
(6) Monitoring.
(c) For facilities that provide reclaimed water for use in open cooling towers, the permittee shall sample the reclaimed water for Cryptosporidium and Giardia. The following requirements shall be met:
1. Sampling shall be conducted at one time during each two-year period. Intervals between sampling shall not exceed two years. This sampling frequency is independent of the treatment plant’s capacity. The sample results shall be submitted to the Department on or before November 28 of each even numbered year using Form 62-610.300(3)(a)4., Pathogen Monitoring, (adopted and incorporated by reference in subparagraph 62-610.300(3)(a)4., F.A.C., effective August 8, 2021, HYPERLINK "http://www.flrules.org/Gateway/reference.asp?No=Ref-13373" http://www.flrules.org/Gateway/reference.asp?No=Ref-13373).
2. Samples shall be taken from at a point immediately following the disinfection process.
3. Samples shall be taken during peak flow periods between the months of August and October.
4. If the wastewater treatment facility uses chlorination for disinfection, samples collected for analysis of Giardia and Cryptosporidium shall be dechlorinated.
5. A grab sample for TSS shall be taken such that it is representative of the water leaving the filters at the treatment facility during the period when pathogen samples are taken. In addition, the permittee shall record the lowest total residual chlorine observed during the period when pathogen samples are collected.
6. EPA Method 1623.1, (adopted and incorporated by reference in subsection 62-610.100(7), F.A.C., effective August 8, 2021, HYPERLINK "http://www.flrules.org/Gateway/reference.asp?No=Ref-133821" http://www.flrules.org/Gateway/reference.asp?No=Ref-133821), or other approved methods in accordance with Rule 62-160.330, F.A.C., for reclaimed water or nonpotable waters, adjusted appropriately to accommodate the detection limit requirements, shall be used. Methods previously allowed for EPA’s ICR shall not be used. The full requirements of the approved method, including quality assurance and quality control, are to be met. Quality assurance and sampling requirements in Chapter 62-160, F.A.C., shall apply.
Two concentrations of Giardia and Cryptosporidium shall be recorded on Part III of Form 62-610.300(3)(a)4., Pathogen Monitoring, (adopted and incorporated by reference in subparagraph 62-610.300(3)(a)4., F.A.C., effective August 8, 2021, HYPERLINK "http://www.flrules.org/Gateway/reference.asp?No=Ref-13373" http://www.flrules.org/Gateway/reference.asp?No=Ref-13373).
a. Total cysts and oocysts shall be enumerated using EPA Method 1623.1, (adopted and incorporated by reference in subsection 62-610.100(7), F.A.C., effective August 8, 2021, HYPERLINK "http://www.flrules.org/Gateway/reference.asp?No=Ref-13382" http://www.flrules.org/Gateway/reference.asp?No=Ref-13382), or other approved methods in accordance with Rule 62-160.330, F.A.C.
b. Potentially viable cysts and oocysts shall be enumerated using the DAPI staining technique contained in EPA Method 1623.1, (adopted and incorporated by reference in subsection 62-610.100(7), F.A.C., effective August 8, 2021, HYPERLINK "http://www.flrules.org/Gateway/reference.asp?No=Ref-13382" http://www.flrules.org/Gateway/reference.asp?No=Ref-13382), or similar enumeration techniques included in other approved methods in accordance with Rule 62-160.330, F.A.C. Cysts and oocysts that are stained DAPI positive or show internal structure by D.I.C. shall be considered as being potentially viable. If the laboratory reports separate values for DAPI positive and for cysts or oocysts having internal structure, the larger of the two concentrations will be reported as being potentially viable.
7. If the number of potentially viable cysts of Giardia reported exceeds 5 per 100 liters or oocysts of Cryptosporidium reported exceeds 22 per 100 liters, a subsequent sample shall be taken and analyzed using EPA Method 1623.1, (adopted and incorporated by reference in subsection 62-610.100(7), F.A.C., effective August 8, 2021, HYPERLINK "http://www.flrules.org/Gateway/reference.asp?No=Ref-13382" http://www.flrules.org/Gateway/reference.asp?No=Ref-13382), or other approved methods in accordance with Rule 62-160.330, F.A.C. This subsequent sample shall be collected within 90 days of the date the initial sample was taken, analyzed for both Giardia and Cryptosporidium, and the results of the subsequent analysis shall be submitted to the department within 60 days of sample collection using Form 62-610.300(3)(a)4., Pathogen Monitoring, (adopted and incorporated by reference in subparagraph 62-610.300(3)(a)4., F.A.C., effective August 8, 2021, HYPERLINK "http://www.flrules.org/Gateway/reference.asp?No=Ref-13373" http://www.flrules.org/Gateway/reference.asp?No=Ref-13373).
Rulemaking Authority 403.051, 403.061, 403.087 FS. Law Implemented 403.021, 403.051, 403.061, 403.062, 403.085, 403.086, 403.087, 403.088 FS. History–New 1-9-96, Amended 8-8-99, 8-8-21.