Fla. Admin. Code R. 62-610.463
(3) Ground water monitoring.
(4) Monitoring for Giardia and Cryptosporidium.
(a) For treatment plants having capacities of 1.0 mgd or larger, the permittee shall sample the reclaimed water for Cryptosporidium and Giardia as follows:
1. Sampling shall be conducted at one time during each two-year period. Intervals between sampling shall not be greater than two years. The sample results shall be submitted to the Department on or before November 28 of that year using Form 62-610.300(3)(a)4., Pathogen Monitoring, (adopted and incorporated by reference in subparagraph 62-610.300(3)(a)4., F.A.C., effective August 8, 2021, HYPERLINK "http://www.flrules.org/Gateway/reference.asp?No=Ref-13373" http://www.flrules.org/Gateway/reference.asp?No=Ref-13373).
2. Samples shall be taken at a point immediately following the disinfection process.
(b) For treatment plants having capacities less than 1.0 mgd, the permittee shall sample the reclaimed water for Cryptosporidium and Giardia as follows:
1. Sampling shall be conducted at one time during each five-year period. Intervals between sampling shall not be greater than five years. The sample results shall be submitted to the Department on or before November 28 of that year using Form 62-610.300(3)(a)4., Pathogen Monitoring, (adopted and incorporated by reference in subparagraph 62-610.300(3)(a)4., F.A.C., effective August 8, 2021, HYPERLINK "http://www.flrules.org/Gateway/reference.asp?No=Ref-13373" http://www.flrules.org/Gateway/reference.asp?No=Ref-13373).
2. Samples shall be taken at a point immediately following the disinfection process.
(f) EPA Method 1623.1, (adopted and incorporated by reference in subsection 62-610.100(7), F.A.C., effective August 8, 2021, HYPERLINK "http://www.flrules.org/Gateway/reference.asp?No=Ref-13382" http://www.flrules.org/Gateway/reference.asp?No=Ref-13382), or other approved methods in accordance with Rule 62-160.330, F.A.C., or other approved methods for reclaimed water or nonpotable waters, adjusted appropriately to accommodate the detection limit requirements, shall be used. Methods previously allowed for EPA’s Information Collection Rule (ICR) shall not be used. The full requirements of the approved method, including quality assurance and quality control, are to be met. Quality assurance and sampling requirements of Chapter 62-160, F.A.C., shall apply.
Two concentrations of Giardia and Cryptosporidium shall be recorded on Part III of Form 62-610.300(3)(a)4., Pathogen Monitoring, (adopted and incorporated by reference in subparagraph 62-610.300(3)(a)4., F.A.C., effective August 8, 2021, HYPERLINK "http://www.flrules.org/Gateway/reference.asp?No=Ref-13373" http://www.flrules.org/Gateway/reference.asp?No=Ref-13373).
1. Total cysts and oocysts shall be enumerated using EPA Method 1623.1, (adopted and incorporated by reference in subsection 62-610.100(7), F.A.C., effective August 8, 2021), HYPERLINK "http://www.flrules.org/Gateway/reference.asp?No=Ref-13382" http://www.flrules.org/Gateway/reference.asp?No=Ref-13382) or other approved methods in accordance with Rule 62-160.330, F.A.C.
2. Potentially viable cysts and oocysts shall be enumerated using the DAPI staining technique contained in EPA Method 1623.1, (adopted and incorporated by reference in subsection 62-610.100(7), F.A.C., effective August 8, 2021, HYPERLINK "http://www.flrules.org/Gateway/reference.asp?No=Ref-13382" http://www.flrules.org/Gateway/reference.asp?No=Ref-13382), or similar enumeration techniques included in other approved methods in accordance with Rule 62-160.330, F.A.C. Cysts and oocysts that are stained DAPI positive or show internal structure by D.I.C. shall be considered as being potentially viable. If the laboratory reports separate values for DAPI positive and for cysts or oocysts having internal structure, the larger of the two concentrations will be reported as being potentially viable.
Rulemaking Authority 403.051, 403.061, 403.087 FS. Law Implemented 403.021, 403.051, 403.061, 403.062, 403.085, 403.086, 403.087, 403.088 FS. History–New 4-4-89, Amended 4-2-90, Formerly 17-610.463, Amended 1-9-96, 8-8-99, 8-8-21.