Fla. Admin. Code R. 62-160.800 – Documents Incorporated by Reference

The following documents, collections and forms are incorporated herein by reference for use in complying with the requirements of this chapter. Except as otherwise indicated below, copies of incorporated documents and forms are available for review during normal business hours at the Department of Environmental Protection, Water Quality Standards Program, 2600 Blair Stone Road, Tallahassee, Florida 32399-2400, or may be obtained from the Department’s website at https://floridadep.gov/dear/quality-assurance, or by writing to the Department of Environmental Protection, Water Quality Standards Program, 2600 Blair Stone Road, MS #6511, Tallahassee, Florida 32399-2400.

(1) Department of Environmental Protection Standard Operating Procedures (DEP SOPs) are organized into the three numbered collections designated below. The DEP SOPs contained in each collection are listed following the title and number of the indicated collection. References in this chapter to the alphanumeric designation for each individual DEP SOP as listed below include reference to all parts, subparts and sections of the cited DEP SOP, unless otherwise cited in a specific rule.
1. (a) Standard Operating Procedures for Field Activities, DEP-SOP-001/01, effective date May 2026:
  1. FA 1000, Regulatory Scope and Administrative Procedures for Use of DEP SOPs, effective date May 2026 (HYPERLINK "https://flrules.org/Gateway/reference.asp?No=Ref-19286"https://flrules.org/Gateway/reference.asp?No=Ref-19286);
  2. FC 1000, Cleaning/Decontamination Procedures, effective date May 2026 (HYPERLINK "https://flrules.org/Gateway/reference.asp?No=Ref-19287"https://flrules.org/Gateway/reference.asp?No=Ref-19287);
  3. FD 1000, Documentation Procedures, effective date May 2026 (HYPERLINK "https://flrules.org/Gateway/reference.asp?No=Ref-19288"https://flrules.org/Gateway/reference.asp?No=Ref-19288);
  4. FM 1000, Field Planning and Mobilization, effective date May 2026 (HYPERLINK "https://flrules.org/Gateway/reference.asp?No=Ref-19289"https://flrules.org/Gateway/reference.asp?No=Ref-19289);
  5. FQ 1000, Field Quality Control Requirements, effective date May 2026 (HYPERLINK "https://flrules.org/Gateway/reference.asp?No=Ref-19290"https://flrules.org/Gateway/reference.asp?No=Ref-19290);
  6. FS 1000, General Sampling Procedures, effective date May 2026 (HYPERLINK "https://flrules.org/Gateway/reference.asp?No=Ref-19291"https://flrules.org/Gateway/reference.asp?No=Ref-19291);
  7. FS 2000, General Aqueous Sampling, effective date May 2026 (HYPERLINK "https://flrules.org/Gateway/reference.asp?No=Ref-19292"https://flrules.org/Gateway/reference.asp?No=Ref-19292);
  8. FS 2100, Surface Water Sampling, effective date May 2026 (HYPERLINK "https://flrules.org/Gateway/reference.asp?No=Ref-19293"https://flrules.org/Gateway/reference.asp?No=Ref-19293);
  9. FS 2200, Groundwater Sampling, effective date May 2026 (HYPERLINK "https://flrules.org/Gateway/reference.asp?No=Ref-19294"https://flrules.org/Gateway/reference.asp?No=Ref-19294);
  10. FS 2300, Drinking Water Sampling, effective date May 2026 (HYPERLINK "https://flrules.org/Gateway/reference.asp?No=Ref-19295"https://flrules.org/Gateway/reference.asp?No=Ref-19295);
  11. FS 2400, Wastewater Sampling, effective date May 2026 (HYPERLINK "https://flrules.org/Gateway/reference.asp?No=Ref-19296"https://flrules.org/Gateway/reference.asp?No=Ref-19296);
  12. FS 3000, Soil Sampling, effective date May 2026 (HYPERLINK "https://flrules.org/Gateway/reference.asp?No=Ref-19297"https://flrules.org/Gateway/reference.asp?No=Ref-19297);
  13. FS 4000, Sediment Sampling, effective date May 2026 (HYPERLINK "https://flrules.org/Gateway/reference.asp?No=Ref-19298"https://flrules.org/Gateway/reference.asp?No=Ref-19298);
  14. FS 5000, Waste Sampling, effective date May 2026 (HYPERLINK "https://flrules.org/Gateway/reference.asp?No=Ref-19299"https://flrules.org/Gateway/reference.asp?No=Ref-19299);
  15. FS 6000, General Biological Tissue Sampling, effective date May 2026 (HYPERLINK "https://flrules.org/Gateway/reference.asp?No=Ref-19300"https://flrules.org/Gateway/reference.asp?No=Ref-19300);
  16. FS 7000, General Biological Community Sampling, effective date May 2026 (HYPERLINK "https://flrules.org/Gateway/reference.asp?No=Ref-19301"https://flrules.org/Gateway/reference.asp?No=Ref-19301);
  17. FS 8100, Contaminated Surface Sampling, effective date May 2026 (HYPERLINK "https://flrules.org/Gateway/reference.asp?No=Ref-19302"https://flrules.org/Gateway/reference.asp?No=Ref-19302);
  18. FS 8200, Clean Sampling for Ultratrace Metals in Surface Waters, effective date May 2026 (HYPERLINK "https://flrules.org/Gateway/reference.asp?No=Ref-19303"https://flrules.org/Gateway/reference.asp?No=Ref-19303);
  19. FT 1000, General Field Testing and Measurement, effective date May 2026 (HYPERLINK "https://flrules.org/Gateway/reference.asp?No=Ref-19304"https://flrules.org/Gateway/reference.asp?No=Ref-19304);
  20. FT 1100, Field Measurement of Hydrogen Ion Activity (pH), effective date May 2026 (HYPERLINK "https://flrules.org/Gateway/reference.asp?No=Ref-19305"https://flrules.org/Gateway/reference.asp?No=Ref-19305);
  21. FT 1200, Field Measurement of Specific Conductance and Conductivity, effective date May 2026 (HYPERLINK "https://flrules.org/Gateway/reference.asp?No=Ref-19306"https://flrules.org/Gateway/reference.asp?No=Ref-19306);
  22. FT 1300, Field Measurement of Salinity, effective date May 2026 (HYPERLINK "https://flrules.org/Gateway/reference.asp?No=Ref-19307"https://flrules.org/Gateway/reference.asp?No=Ref-19307);
  23. FT 1400, Field Measurement of Temperature, effective date May 2026 (HYPERLINK "https://flrules.org/Gateway/reference.asp?No=Ref-19308"https://flrules.org/Gateway/reference.asp?No=Ref-19308);
  24. FT 1500, Field Measurement of Dissolved Oxygen, effective date May 2026 (HYPERLINK "https://flrules.org/Gateway/reference.asp?No=Ref-19309"https://flrules.org/Gateway/reference.asp?No=Ref-19309);
  25. FT 1600, Field Measurement of Turbidity and Total Suspended Solids (TSS), effective date May 2026 (HYPERLINK "https://flrules.org/Gateway/reference.asp?No=Ref-19310"https://flrules.org/Gateway/reference.asp?No=Ref-19310);
  26. FT 1700, Field Measurement of Light Penetration (Secchi Depth and Transparency), effective date May 2026 (HYPERLINK "https://flrules.org/Gateway/reference.asp?No=Ref-19311"https://flrules.org/Gateway/reference.asp?No=Ref-19311);
  27. FT 1800, Field Measurement of Stage, Surface Water Velocity, and Discharge (Flow) and Computations of Streamflow, effective date May 2026 (HYPERLINK "https://flrules.org/Gateway/reference.asp?No=Ref-19312"https://flrules.org/Gateway/reference.asp?No=Ref-19312);
  28. FT 1900, Continuous Monitoring Meters, effective date May 2026 (HYPERLINK "https://flrules.org/Gateway/reference.asp?No=Ref-19313"https://flrules.org/Gateway/reference.asp?No=Ref-19313)
  29. FT 2000, Field Measurement of Residual Chlorine, effective date May 2026 (HYPERLINK "https://flrules.org/Gateway/reference.asp?No=Ref-19314"https://flrules.org/Gateway/reference.asp?No=Ref-19314);
  30. FT 2100, Field Measurement of Nitrate Using Ultraviolet Optical Sensors, effective date May 2026 (HYPERLINK "https://flrules.org/Gateway/reference.asp?No=Ref-19315"https://flrules.org/Gateway/reference.asp?No=Ref-19315); and,
  31. FT 3000, Aquatic Habitat Characterization, effective date May 2026 (HYPERLINK "https://flrules.org/Gateway/reference.asp?No=Ref-19316"https://flrules.org/Gateway/reference.asp?No=Ref-19316).
2. (b) Standard Operating Procedures for Laboratory Activities, DEP-SOP-002/01, effective date May 2026:
  1. LD 1000, Laboratory Documentation, effective date May 2026 (HYPERLINK "https://flrules.org/Gateway/reference.asp?No=Ref-19317"https://flrules.org/Gateway/reference.asp?No=Ref-19317);
  2. LQ 1000, Laboratory Quality Control, effective date May 2026 (HYPERLINK "https://flrules.org/Gateway/reference.asp?No=Ref-19318"https://flrules.org/Gateway/reference.asp?No=Ref-19318); and,
  3. LT 7000, Determination of Biological Indices, effective date May 2026 (HYPERLINK "https://flrules.org/Gateway/reference.asp?No=Ref-19319"https://flrules.org/Gateway/reference.asp?No=Ref-19319).
3. (c) Standard Operating Procedures for Selected Bioassessment Activities, DEP-SOP-003/11, effective date May 2026:
  1. BRN 1000, Biological Reconnaissance Field Method, effective date May 2026 (HYPERLINK "https://flrules.org/Gateway/reference.asp?No=Ref-19320"https://flrules.org/Gateway/reference.asp?No=Ref-19320);
  2. LVI 1000, Lake Vegetation Index Methods, effective date May 2026 (HYPERLINK "https://flrules.org/Gateway/reference.asp?No=Ref-19321"https://flrules.org/Gateway/reference.asp?No=Ref-19321); and,
  3. SCI 1000, Stream Condition Index Methods, effective date May 2026 (HYPERLINK "https://flrules.org/Gateway/reference.asp?No=Ref-19322"https://flrules.org/Gateway/reference.asp?No=Ref-19322).
(2) The following documents and DEP forms are cited in certain DEP SOPs included in the numbered collections DEP-SOP-001/01, DEP-SOP-002/01 or DEP-SOP-003/11 (citation locations in parentheses).
1. (a) Methods and other documents published by the United States Environmental Protection Agency (EPA), as listed below.
  1. EPA Method 5035A, Revision 1, July 2002, in SW-846, Test Methods for Evaluating Solid Waste, Physical/Chemical Methods (DEP-SOP-001/01) ( HYPERLINK "http://www.flrules.org/Gateway/reference.asp?No=Ref-07984" http://www.flrules.org/Gateway/reference.asp?No=Ref-07984);
  2. EPA Method 1623.1, Cryptosporidium and Giardia in Water by Filtration/IMS/FA, EPA 816-R-12-001, January 2012 (DEP-SOP-001/01) ( HYPERLINK "http://www.flrules.org/Gateway/reference.asp?No=Ref-07985" http://www.flrules.org/Gateway/reference.asp?No=Ref-07985); and,
  3. U.S. EPA ICR Microbial Laboratory Manual, EPA/600/R-95/178, April 1996, Section VII, Part 9, Sampling (DEP-SOP-001/01) ( HYPERLINK "http://www.flrules.org/Gateway/reference.asp?No=Ref-04338" http://www.flrules.org/Gateway/reference.asp?No=Ref-04338).
2. (b) Code of Federal Regulations (CFR):
  1. Table II, Required Containers, Preservation Techniques, and Holding Times, 40 CFR 136.3(e), revised as of July 1, 2024, effective date May 2026, including all footnotes (DEP-SOP-001/01) (HYPERLINK "https://flrules.org/Gateway/reference.asp?No=Ref-19323"https://flrules.org/Gateway/reference.asp?No=Ref-19323); and,
  2. 29 CFR 1910.120, Hazardous Waste Operations and Emergency Response, revised as of July 1, 2024, effective date May 2026 (DEP-SOP-001/01) (HYPERLINK "https://flrules.org/Gateway/reference.asp?No=Ref-19324"https://flrules.org/Gateway/reference.asp?No=Ref-19324).
3. (c) Methods and sections included in Standard Methods for the Examination of Water and Wastewater, published by American Public Health Association (APHA), as listed below. These documents are copyright protected. Copies of these documents are available for review during normal business hours at the Department of Environmental Protection, Water Quality Standards Program, 2600 Blair Stone Road, Tallahassee, Florida 32399-2400, or at the Department of State, R.A. Gray Building, 500 South Bronough Street, Tallahassee, Florida 32399-0250, or from the publisher at APHA, 800 I Street NW, Washington, DC 20001-3710, or from the publisher at https://www.standardmethods.org/.
  1. Section 1060, Collection and Preservation of Samples, subsection 1060 A.2., Safety Considerations 2011 (DEP-SOP-001/01);
  2. Section 9060, Samples, subsection 9060 A.3.a., Potable Water, 2013 (DEP-SOP-001/01), effective date May 2026;
  3. Method 2130 B, Turbidity, section 3., Reagents, 2011 (DEP-SOP-001/01);
  4. Method 2510, Conductivity, 2011 (DEP-SOP-001/01);
  5. Method 2520, Salinity, 2011 (DEP-SOP-001/01);
  6. Methods 4500-Cl B, C, D, E, F, and G, 2011 (DEP-SOP-001/01);
  7. Methods 4500-O B, C, H, and G, Oxygen (Dissolved), 2016 (DEP-SOP-001/01), effective date May 2026;
  8. Table 4500-H+:2, Preparation of pH Standard Solutions, in method 4500-H+-B, (2021) (DEP-SOP-001/01), effective date May 2026; and,
  9. Method 4500-NO3-B, Nitrogen (Nitrate) Ultraviolet Spectrophotometric Screening Method, 2016 (DEP-SOP-001/01), effective date May 2026.
4. (d) ASTM E1391-03 (2008), Standard Guide for Collection, Storage, Characterization, and Manipulation of Sediments for Toxicological Testing and for Selection of Samplers Used to Collect Benthic Invertebrates, 2003, ASTM International (DEP-SOP-001/01), effective date May 2026. This document is copyright protected. A copy of this document is available for review during normal business hours at the Department of Environmental Protection, Water Quality Standards Program, 2600 Blair Stone Road, Tallahassee, Florida 32399-2400, or at the Department of State, R.A. Gray Building, 500 South Bronough Street, Tallahassee, Florida 32399-0250, or from the publisher at ASTM International, 100 Barr Harbor Drive, P.O. Box C700, West Conshohocken, PA 19428-2959, or from the publisher’s website at http://www.astm.org/Standard/index.html.
5. (e) Sampling and Use of the Stream Condition Index (SCI) for Assessing Flowing Waters: A Primer, DEP-SAS-001/11, effective date May 2026 (DEP-SOP-003/11) (HYPERLINK "https://flrules.org/Gateway/reference.asp?No=Ref-19325"https://flrules.org/Gateway/reference.asp?No=Ref-19325).
6. (f) Sampling and Use of the Lake Vegetation Index (LVI) for Assessing Lake Plant Communities in Florida: A Primer, DEP-SAS-002/11, effective date May 2026 (DEP-SOP-003/11) (HYPERLINK "https://flrules.org/Gateway/reference.asp?No=Ref-19326"https://flrules.org/Gateway/reference.asp?No=Ref-19326).
7. (g) United States Geological Survey, Temperature: U.S. Geological Survey Techniques and Methods, book 9, chap. A6.1, 14 p., 2024, effective date May 2026 (DEP-SOP-001/01) (HYPERLINK "https://flrules.org/Gateway/reference.asp?No=Ref-19327"https://flrules.org/Gateway/reference.asp?No=Ref-19327 HYPERLINK "https://www.flrules.org/Gateway/reference.asp?No=Ref-04343" ).
8. (h) DEP Forms cited:
  1. Form FD 9000-5, Stream/River Habitat Assessment Field Sheet, effective date May 2026 (DEP-SOP-001/01, DEP-SOP-003/11) (HYPERLINK "https://flrules.org/Gateway/reference.asp?No=Ref-19328"https://flrules.org/Gateway/reference.asp?No=Ref-19328);
  2. Form FD 9000-6, Lake Habitat Assessment Field Sheet, effective date May 2026 (DEP-SOP-001/01) (HYPERLINK "https://flrules.org/Gateway/reference.asp?No=Ref-19329"https://flrules.org/Gateway/reference.asp?No=Ref-19329);
  3. Form FD 9000-34, Stream Habitat Assessment Training and Evaluation Checklist and Event Log, effective date May 2026 (DEP-SOP-001/01) (HYPERLINK "https://flrules.org/Gateway/reference.asp?No=Ref-19330"https://flrules.org/Gateway/reference.asp?No=Ref-19330); and,
  4. Form FD 9000-35, Stream Condition Index and BioRecon Training Checklist and Event Log, effective date May 2026 (DEP-SOP-003/11) (HYPERLINK "https://flrules.org/Gateway/reference.asp?No=Ref-19331"https://flrules.org/Gateway/reference.asp?No=Ref-19331).
9. (i) United States Geological Survey (USGS) references included in DEP SOP FT 1800, Field Measurement of Stage, Surface Water Velocity, and Discharge (Flow) and Computations of Streamflow, incorporated by reference in subparagraph 62-160.800(1)(a)27., F.A.C., effective date May 2026, (DEP-SOP-001/01). Copies of these documents may be obtained from the United States Geological Survey, 12201 Sunrise Valley Drive, Reston, VA 20192, or through USGS’s website at https://pubs.usgs.gov/.
  1. United States Geological Survey, Techniques of Water-Resources Investigations, Book 3, https://pubs.usgs.gov/twri/index090905.html:
  a. Chapter A2, Dalrymple, Tate, and Benson, M.A., 1968, Measurement of peak discharge by the slope-area method, effective date May 2026 (HYPERLINK "https://flrules.org/Gateway/reference.asp?No=Ref-19354"https://flrules.org/Gateway/reference.asp?No=Ref-19354);
  b. Chapter A3, Bodhaine, G.L, 1968, Measurement of peak discharge at culverts by indirect methods, effective date May 2026 (HYPERLINK "https://flrules.org/Gateway/reference.asp?No=Ref-19355"https://flrules.org/Gateway/reference.asp?No=Ref-19355);
  c. Chapter A4, Muttahai, H.F. 1967, Measurement of peak discharge at width contractions by indirect methods, effective date May 2026 (HYPERLINK "https://flrules.org/Gateway/reference.asp?No=Ref-19356"https://flrules.org/Gateway/reference.asp?No=Ref-19356);
  d. Chapter A5, Hulsing, Harry, 1967, Measurement of peak discharge at dams by indirect method, effective date May 2026 (HYPERLINK "https://flrules.org/Gateway/reference.asp?No=Ref-19357"https://flrules.org/Gateway/reference.asp?No=Ref-19357);
  e. Chapter A6, Carter, R.W., and Davidian, Jacob, 1968, General procedure for gaging streams, effective date May 2026 (HYPERLINK "https://flrules.org/Gateway/reference.asp?No=Ref-19358"https://flrules.org/Gateway/reference.asp?No=Ref-19358);
  f. Chapter A10, Kennedy, E.J., 1984, Discharge ratings at gaging stations, effective date May 2026 (HYPERLINK "https://flrules.org/Gateway/reference.asp?No=Ref-19359"https://flrules.org/Gateway/reference.asp?No=Ref-19359);
  g. Chapter A11, Smoot, G.F., and Novack, C.E., 1969, Measurement of discharge by the moving-boat method, effective date May 2026 (HYPERLINK "https://flrules.org/Gateway/reference.asp?No=Ref-19360"https://flrules.org/Gateway/reference.asp?No=Ref-19360);
  h. Chapter A13, Kennedy, E.J., 1983, Computation of continuous records of streamflow, effective date May 2026 (HYPERLINK "https://flrules.org/Gateway/reference.asp?No=Ref-19361"https://flrules.org/Gateway/reference.asp?No=Ref-19361);
  i. Chapter A14, Kilpatrick, F.A. and Schneider, V.R., 1983, Use of flumes in measuring discharge, effective date May 2026 (HYPERLINK "https://flrules.org/Gateway/reference.asp?No=Ref-19362"https://flrules.org/Gateway/reference.asp?No=Ref-19362); and,
  j. Chapter A21, Wagner, C. Russell, 1995, Stream-gaging cableways, effective date May 2026 (HYPERLINK "https://flrules.org/Gateway/reference.asp?No=Ref-19363"https://flrules.org/Gateway/reference.asp?No=Ref-19363).
  2. United States Geological Survey, Techniques and Methods, Book 3, https://pubs.usgs.gov/browse/Report/USGS%20Numbered%20Series/Techniques%20and%20Methods/:
  a. Chapter A7, Sauer, V.B., and Turnipseed, D.P., 2010, Stage measurement at gaging stations, effective date May 2026 (HYPERLINK "https://flrules.org/Gateway/reference.asp?No=Ref-19364"https://flrules.org/Gateway/reference.asp?No=Ref-19364);
  b. Chapter A8, Turnipseed, D.P., and Sauer, V.B., 2010, Discharge measurements at gaging stations, effective date May 2026 (HYPERLINK "https://flrules.org/Gateway/reference.asp?No=Ref-19365"https://flrules.org/Gateway/reference.asp?No=Ref-19365);
  c. Chapter A22, Mueller, D.S., Wagner, C.R., Rimmel, M.S., Oberg, K.A., and Rainville, Francois, 2013, Measuring discharge with acoustic Doppler current profilers from a moving boat, effective date May 2026 (HYPERLINK "https://flrules.org/Gateway/reference.asp?No=Ref-19366"https://flrules.org/Gateway/reference.asp?No=Ref-19366); and,
  d. Chapter A23, Levesque, V.A., and Oberg, K.A., 2012, Computing discharge using the index velocity method, effective date May 2026 (HYPERLINK "https://flrules.org/Gateway/reference.asp?No=Ref-19367"https://flrules.org/Gateway/reference.asp?No=Ref-19367).
(3) NELAC and TNI Standards, as listed below. These documents are copyright protected. Copies of these documents are available for review during normal business hours at the Department of Environmental Protection, Water Quality Standards Program, 2600 Blair Stone Road, Tallahassee, Florida 32399-2400, or at the Department of State, R.A. Gray Building, 500 South Bronough Street, Tallahassee, Florida 32399-0250, or from the publisher at The NELAC Institute, P.O. Box 2439, Weatherford, TX 76086, or the publisher’s website at http://www.nelac-institute.org/index.php.
1. (a) Glossary, Appendix A to Chapter 1, Program Policy and Structure, 2003 NELAC Standards, Approved June 5th, 2003, EPA/600/R-04/003 ( HYPERLINK "http://www.flrules.org/Gateway/reference.asp?No=Ref-04357" http://www.flrules.org/Gateway/reference.asp?No=Ref-04357); and,
2. (b) The NELAC Institute (TNI), EL-V1-ISO-2016-Rev. 2.1, Environmental Laboratory Sector, Vol. 1, Modules 1-7, Management and Technical Requirements for Laboratories Performing Environmental Analysis (2016), effective date May 2026. Referred to as “2016 TNI Standard” in this chapter and incorporated documents.
(4) Quality Assurance Project Plan Standard, EPA CIO 2105-S-02.1, Form Rev. 08/21/2023, effective date May 2026 (HYPERLINK "https://flrules.org/Gateway/reference.asp?No=Ref-19333"https://flrules.org/Gateway/reference.asp?No=Ref-19333).
(5) Alternative and Modified Analytical Laboratory Methods, DEP-QA-001/01, effective date May 2026 (HYPERLINK "https://flrules.org/Gateway/reference.asp?No=Ref-19334"https://flrules.org/Gateway/reference.asp?No=Ref-19334) and the following documents cited therein:
1. (a) Definition and Procedure for the Determination of the Method Detection Limit – Revision 2, 40 CFR Part 136, Appendix B, revised as of July 1, 2024, effective date May 2026 (HYPERLINK "https://flrules.org/Gateway/reference.asp?No=Ref-19335"https://flrules.org/Gateway/reference.asp?No=Ref-19335);
2. (b) IUPAC – Nomenclature in Evaluation of Analytical Methods including Detection and Quantification Capabilities, Pure & Appl. Chem., Vol. 67, No. 10, pp. 1699-1723, ©1995 ( HYPERLINK "http://www.flrules.org/Gateway/reference.asp?No=Ref-04361" http://www.flrules.org/Gateway/reference.asp?No=Ref-04361); available for download at http://www.iupac.org/publications/pac/index/ or available for review during normal business hours at the Department of Environmental Protection, Water Quality Standards Program, 2600 Blair Stone Road, Tallahassee, Florida 32399-2400; and,
3. (c) Hubaux, A., G. Vos, Decision and Detection Limits for Linear Calibration Curves, Analytical Chemistry, Vol. 42. No. 8, pp. 849-855, July 1970; available for download at http://pubs.acs.org/journal/ancham or available for review during normal business hours at the Department of Environmental Protection, Water Quality Standards Program, 2600 Blair Stone Road, Tallahassee, Florida 32399-2400.
(6) 40 CFR 136.3, Identification of test procedures, revised as of July 1, 2024, effective date May 2026 (HYPERLINK "https://flrules.org/Gateway/reference.asp?No=Ref-19336"https://flrules.org/Gateway/reference.asp?No=Ref-19336).
(7) 40 CFR 136.4, Application for and approval of alternate test procedures for nationwide use, revised as of July 1, 2024, effective date May 2026 (HYPERLINK "https://flrules.org/Gateway/reference.asp?No=Ref-19337"https://flrules.org/Gateway/reference.asp?No=Ref-19337).
(8) 40 CFR 136.5, Approval of alternate test procedures for limited use, revised as of July 1, 2024, effective date May 2026 (HYPERLINK "https://flrules.org/Gateway/reference.asp?No=Ref-19340"https://flrules.org/Gateway/reference.asp?No=Ref-19340).
(9) 40 CFR 136.6, Method modifications and analytical requirements, revised as of July 1, 2024 (HYPERLINK "https://flrules.org/Gateway/reference.asp?No=Ref-19338"https://flrules.org/Gateway/reference.asp?No=Ref-19338), effective date May 2026.
(10) 40 CFR 141.27, Alternate analytical techniques, revised as of July 1, 2024, effective date May 2026 (HYPERLINK "https://flrules.org/Gateway/reference.asp?No=Ref-19339"https://flrules.org/Gateway/reference.asp?No=Ref-19339).
(11) Chapter Two, Choosing the Correct Procedure, Section 2.1, Guidance Regarding Flexibility Inherent to SW-846 Methods and the Precedence of SW-846 Quality Control Criteria (July 2014), in Test Methods for Evaluating Solid Waste, Physical/Chemical Methods (SW-846), effective date May 2026 (HYPERLINK "https://flrules.org/Gateway/reference.asp?No=Ref-19341"https://flrules.org/Gateway/reference.asp?No=Ref-19341). This publication may be viewed at https://www.epa.gov/hw-sw846/sw-846-compendium, where it may also be downloaded. A printed copy may be obtained from the National Technical Information Service, U.S. Department of Commerce, 5301 Shawnee Road, Alexandria, VA. 22312.
(12) Guidelines for Collaborative Study Procedures to Validate Characteristics of a Method of Analysis, Appendix D, Official Methods of Analysis of AOAC INTERNATIONAL, 19th edition (2012). A copy of this document is available for review during normal business hours at the Department of Environmental Protection, Water Quality Standards Program, 2600 Blair Stone Road, Tallahassee, Florida 32399-2400 or from the publisher at AOAC INTERNATIONAL, 481 N. Frederick Ave., Suite 500, Gaithersburg, MD 20877, or from the publisher’s website at http://www.aoac.org.
(13) Process for Assessing Data Usability (DEP-EA-001/07), effective date May 2026 (HYPERLINK "https://flrules.org/Gateway/reference.asp?No=Ref-19342"https://flrules.org/Gateway/reference.asp?No=Ref-19342).
(14) Applicability of Chlorophyll a Methods (DEP-SAS-002/10), effective date May 2026 (HYPERLINK "https://flrules.org/Gateway/reference.asp?No=Ref-19343"https://flrules.org/Gateway/reference.asp?No=Ref-19343), and the following documents cited therein:
1. (a) Standard Methods for the Examination of Water and Wastewater, Method 10150, Determination of Chlorophyll A, Sections B, C, and D, 2022, published by APHA, effective date May 2026. This document is copyright protected. A copy of this document is available for review during normal business hours at the Department of Environmental Protection, Water Quality Standards Program, 2600 Blair Stone Road, Tallahassee, Florida 32399-2400, or at the Department of State, R.A. Gray Building, 500 South Bronough Street, Tallahassee, Florida 32399-0250, or from the publisher at APHA, 800 I Street NW, Washington, DC 20001-3710, or from the publisher at https://www.standardmethods.org/buy/.
2. (b) EPA 445.0, In Vitro Determination of Chlorophyll a and pheophytin a in Marine and Freshwater Algae by Fluorescence, Rev. 1.2, September 1997, effective date May 2026 (HYPERLINK "https://flrules.org/Gateway/reference.asp?No=Ref-19344"https://flrules.org/Gateway/reference.asp?No=Ref-19344).
3. (c) EPA 446.0, In Vitro Determination of Chlorophylls a, b, c1 + c2 and Pheopigments in Marine and Freshwater Algae by Visible Spectrophotometry, Rev. 1.2, September 1997, effective date May 2026 (HYPERLINK "https://flrules.org/Gateway/reference.asp?No=Ref-19345"https://flrules.org/Gateway/reference.asp?No=Ref-19345).
4. (d) EPA 447.0, Determination of Chlorophylls a and b and Identification of Other Pigments of Interest in Marine and Freshwater Algae Using High Performance Liquid Chromatography with Visible Wavelength Detection, Version 1.0, September 1997, effective date May 2026 (HYPERLINK "https://flrules.org/Gateway/reference.asp?No=Ref-19346"https://flrules.org/Gateway/reference.asp?No=Ref-19346).
5. (e) Standard Methods for the Examination of Water and Wastewater, Method 10200 H., Determination of Chlorophyll A, Sections 2, 3, and 4, 2011, published by APHA, effective date May 2026. This document is copyright protected. A copy of this document is available for review during normal business hours at the Department of Environmental Protection, Water Quality Standards Program, 2600 Blair Stone Road, Tallahassee, Florida 32399-2400, or at the Department of State, R.A. Gray Building, 500 South Bronough Street, Tallahassee, Florida 32399-0250, or from the publisher at APHA, 800 I Street NW, Washington, DC 20001-3710, or from the publisher at https://www.standardmethods.org/buy/.
(15) Chapter 64E-1, F.A.C., “Certification of Environmental Testing Laboratories,” effective September 26, 2018, (HYPERLINK "https://flrules.org/Gateway/reference.asp?No=Ref-19347"https://flrules.org/Gateway/reference.asp?No=Ref-19347).

Rulemaking Authority 403.061, 403.0623 FS. Law Implemented 373.026, 373.309, 373.409, 373.413, 373.414, 373.416, 373.4592, 376.303, 376.305, 376.3071, 403.0623, 403.0625, 403.087, 403.088, 403.0881, 403.504, 403.704, 403.707, 403.722, 403.853 FS. History–New 4-9-02, Amended 6-8-04, 12-3-08, 7-30-14, 4-16-18, 5-20-26.