49 C.F.R. § 40.189
Link to an amendment published at 82 FR 52245, Nov. 13, 2017.
You can find more information concerning split specimens in several sections of this part:
§ 40.3 - Definition. § 40.65 - Quantity of split specimen. § 40.67 - Directly observed test when split specimen is unavailable. §§ 40.71-40.73 - Collection process for split specimens. § 40.83 - Laboratory accessioning of split specimens. § 40.99 - Laboratory retention of split specimens. § 40.103 - Blind split specimens. § 40.153 - MRO notice to employees on tests of split specimen. §§ 40.193 and 40.201 - MRO actions on insufficient or unavailable split specimens. Appendix D to Part 40 - Report format for split specimen failure to reconfirm.