The specific tests and test organisms used for determining whole effluent toxicity include:

1. (1) Acute test/D. pulex. Acute 48-hour static renewal toxicity test using Daphia pulex (Method 2021.0) as described in EPA publication no. 821-R-02-012 (October 2002), Fifth Edition, Methods for Measuring the Acute Toxicity of Effluent to Freshwater and Marine Organisms.
2. (2) Acute test/P. promelas. Acute 48-hour static renewal toxicity test using Pimephales promelas (Method 2000.0) as described in EPA publication no. 821-R-02-012 (October 2002), Fifth Editon, Methods for Measuring the Acute Toxicity of Effluent to Freshwater and Marine Organisms.
3. (3) Chronic test/C. dubia. Chronic 7-day static renewal survival and reproduction test using Ceriodaphnia dubia (Method 1002.0), as described in EPA publication no. 821-R-02-013 (October 2002), Fourth Edition, Short Term Methods for Estimating the Chronic Toxicity of Effluents and Receiving Waters to Freshwater Organisms.
4. (4) Chronic test/P. promelas. Chronic 7-day static renewal larval survival and growth test using Pimephales promelas (fathead minnow) (Method 1000.0), as described in EPA publication no. 821-R-02-013 (October 2002), Fourth Edition, Short Term Methods for Estimating the Chronic Toxicity of Effluents and Receiving Waters to Freshwater Organisms.
5. (5) Acute test/D. Magna. Acute 48-hour static renewal toxicity test using Daphnia magna (Method 2021.0), as described in EPA Publication No. 821-R-02-012 (October, 2002), fifth edition, Methods for Measuring the Acute Toxicity of Effluent to Freshwater and Marine Organisms.
6. (6) Chronic test/D. Magna. Chronic 21-day static renewal life-cycle toxicity test using Daphnia magna, (ASTM Designation E-1193), as described in ASTM Publication E-1193-97 (Reapproved 2004), Standard Guide for Conducting Daphnia Magna Life Cycle Toxicity Tests.
7. (7) Acute test/Mussels. Acute toxicity test using mussels (ASTM Designation E-2455) as described in ASTM Publication E-2455-06 (Approved April 2006), Standard Guide for Conducting Laboratory Toxicity Test with Freshwater Mussels.

Added at 41 Ok Reg, Number 23, effective 9-15-24