1. (1) Unit of certification. The unit of certification shall be a field. Parts of a field shall not be eligible for certification.
2. (2) Isolation requirement. A specific commercial hybrid to be inspected for certification must be so located that it is not less than one mile away from any other varieties of any species in bloom at the same time as the seed field. This requirement shall not apply to small garden areas with less than 50 plants providing such garden areas are not within 1,000 feet of the seed production field.

3. (3) Pollinating insect requirement. In order to insure adequate cross pollination there shall be at least two colonies of bees per acre preferably located in or immediately adjacent to the field boundary. In no case shall the bees be located more than 1,000 feet from a boundary of the field.

   (b) Specific requirements.

4. (1) Offtype plants.

   1. (i) Plant spacing to permit inspection of the crop for offtype plants. The total crop must be thinned to single plants with the pollen parent spaced no closer than six inches between plants and the seed parent spaced no closer than 15 inches between plants.
   2. (ii) Pollen parent. On any one inspection there shall not be more than four offtype plants per acre of pollen parent. (Approximately one offtype in 5,000 plants.)

   3. (iii) Seed parent. On any one inspection there shall not be more than three offtype plants per acre of seed parent. (Approximately one offtype in 2,000 plants.) Note:

      Note:

      In determining the number of offtype plants for this purpose, only those shall be counted on which the male flowers are in bloom, or have bloomed during the seed parent debudding period.

5. (2) Debudding. On any one inspection during the debudding period, there shall not be more than an average of two male flowers which have bloomed per 100 seed parent plants. The total male flowers for three or more inspections shall not exceed 85 per cent of the number of inspections times the maximum tolerance for one inspection.
6. (3) Virus. On any one inspection prior to the final inspection the total number of plants infected with squash mosaic and/or other seed-borne viruses shall not exceed 0.01 per cent, nor shall the total number of plants infected by viruses other than those known to be seed-borne exceed 1.0 per cent. On the final fruit inspection the total of all virus infected fruit shall be none. Any fruit found showing evidence of virus infection shall be removed and destroyed in the presence of the inspector.

(a) General.