- A. Test result data for use by the Louisiana Molluscan Shellfish Program shall be generated by an officially designated laboratory of the Louisiana molluscan shellfish sanitation laboratory system.
B. Determination of results of microbiological analysis of shellfish growing waters shall conform to criteria stipulated in the current edition of American Public Health Association (APHA) Recommended Procedures for the Examination of Sea Water and Shellfish and appropriate sections in Official Methods of Analysis, of the Association of Official Analytical Chemists (A.O.A.C.).
- 1. The presence of gas in the inverted vial or of dissolved gas which can be removed by slight agitation of the test medium test tube shall constitute a positive test. The number of positive tests in each dilution shall be recorded for determination of the Most Probable Number (MPN).
- 2. The standard Most Probable Number (MPN) tables as found in the appropriate tables in the current edition of American Public Health Association (APHA) Recommended Procedures for the Examination of Sea Water and Shellfish and Official Methods of Analysis, of the Association of Official Analytical Chemists (A.O.A.C.) shall be used to determine MPN values.
- C. All test result data shall be verified and documented and shall be reported by the laboratory as fecal coliform MPN/100 ml sample to the proper authorities.
- D. A record of all test result data shall be maintained by the laboratory or remain accessible to the laboratory for a period of five years. Records may be in tabular and/or electronic form and should include date, place and time of sampling, name of person collecting sample, identification of sample, date of receipt of sample and analysis, laboratory person responsible for performing analysis, analytical technique used, and results of analysis.
Authority Note
AUTHORITY NOTE: Promulgated in accordance with R.S. 40:4.A.(1) , R.S. 40:5.A(2)(3)(5)(7)(9)(15)(17)(19)(20) and R.S. 40:5.3.
Historical Note
HISTORICAL NOTE: Promulgated by the Department of Health and Hospitals, Office of Public Health, LR 28:1296 (June 2002), amended by the Department of Health, Office of Public Health, LR 48:1848 (July 2022), repromulgated LR 48:2136 (August 2022).