1. A. Start Up Phase. Prior to certification of a depuration plant, the following minimum sampling schedule shall be followed in order to verify the effectiveness of the treatment process:

   1. 1. One or more shellfish samples (12 or more shellfish per sample) shall be collected for bacterial examination before the shellfish are submitted to the treatment process.
   2. 2. Three or more shellfish samples, (12 or more shellfish per sample) randomly selected from three or more locations in each tank, shall be collected for bacterial examination after 24 hours of depuration.
   3. 3. Three or more shellfish samples, (12 or more shellfish per sample) randomly selected from three or more locations in each tank, shall be collected for bacterial examination after the shellfish have completed the treatment process.
2. B. The above schedule shall be followed until such time as the Department of Health and Hospitals, after review of the results, determines that the shellfish from such areas are responding properly to the treatment process, and that the process is successfully reducing bacterial levels. After such a determination, a routine sampling procedure shall be followed that conforms with the requirements of the latest edition of the National Shellfish Sanitation Program Manual of Operations, Part II. Written permission from the Department of Health and Hospitals must be obtained prior to the initiation of routine monitoring procedures. A bacteriological sampling program shall not be required for shellfish undergoing wet storage

3. C. In the event of the installation of a new laboratory, new laboratory equipment, employment of new laboratory personnel, initiation of new laboratory procedures, or the alteration of treatment or procedures, the Department of Health and Hospitals may require reinitiation of "start-up phase" procedures until such time as the department, after review of the results, determines that the laboratory and/or treatment procedures are providing valid results. Written permission from the department shall be obtained before routine monitoring procedures are again followed.

   §1229. Depuration and/or Wet Storage Process Water ControlSampling
   [Formerly 49:6.1150]

4. A. All controlled processes require quality assurance testing to determine if standards are being met and if controls are effective. The treatment or wet storage of shellfish is a controlled process designed to reduce and/or maintain bacterial contamination to an acceptable level. To insure the continuing effectiveness of the shellfish treatment process, the minimum sampling procedure as described below, shall be followed:

5. B. Incoming Sea Water

   1. 1. Type of Test: temperature, turbidity, salinity, dissolved oxygen, bacteriological.
   2. 2. Frequency: prior to initiation of 48-hour treatment or wet storage process.

6. C. Effluent from Ultraviolet Light Treatment Unit

   1. 1. Type of Test: bacteriological
   2. 2. Frequency: once per day per unit.

Authority Note

AUTHORITY NOTE: Promulgated in accordance with Louisiana Revised Statutes of 1950, Title 40, as amended.

Historical Note

HISTORICAL NOTE: Promulgated by the Department of Health and Human Resources, Office of Preventive and Public Health Services, LR 11:550 (May 1985), amended by the Department of Health and Hospitals, Office of Public Health LR16:414 (May 1990).

Authority Note

AUTHORITY NOTE: Promulgated in accordance with Louisiana Revised Statutes of 1950, Title 40, as amended.

Historical Note

HISTORICAL NOTE: Promulgated by the Department of Health and Human Resources, Office of Preventive and Public Health Services, LR 11:550 (May 1985), amended by the Department of Health and Hospitals, Office of Public Health LR16:414 (May 1990).