Authority: IC 4-31-3-9

Affected: IC 4-31-12

Sec. 8. (a) No AAS (androgenic-anabolic steroid) shall be permitted in test samples collected from racing horses except for endogenous concentrations of the naturally occurring substances boldenone, nandrolone, and testosterone at concentrations less than the indicated thresholds.

(b) Concentrations of these AAS shall not exceed the following urine threshold concentrations for total (i.e., free drug or metabolite and drug or metabolite liberated from its conjugates) steroid:

1. (1) Boldenone:

   1. (A) In male horses other than geldings – fifteen (15) ng/ml of urine.
   2. (B) In geldings, fillies, and mares – one (1) ng/mL of urine.

2. (2) Nandrolone:

   1. (A) In geldings - one (1) ng/ml of urine.
   2. (B) In fillies and mares – one (1) ng/ml of urine.
   3. (C) In male horses other than geldings – forty-five (45) ng/ml of nandrolone metabolite, 5a-estrane-3ß,17a- diol of urine.

3. (3) Testosterone:

   1. (A) In geldings – twenty (20) ng/ml of urine.
   2. (B) In fillies and mares – fifty-five (55) ng/ml of urine, unless in foal.
   3. (C) In male horses other than geldings minimum thresholds will not apply.

(c) Concentrations of these AAS shall not exceed the following free (i.e., not conjugated) steroid concentrations in plasma or serum:

1. (1) Boldenone: For all horses a confirmatory threshold not greater than 25 pg/ml shall apply.

2. (2) Nandrolone:

   1. (A) In geldings, fillies, and mares – a confirmatory threshold not greater than 25 pg/ml shall apply.
   2. (B) In male horses other than geldings – nandrolone shall be tested for in urine only.

3. (3) Testosterone:

   1. (A) In geldings, fillies, and mares – a confirmatory threshold not greater than 25 pg/ml.
   2. (B) In male horses other than geldings minimum thresholds will not apply.

(d) All other AAS are prohibited in racing horses.

(e) The sex of the horse must be identified to the laboratory for all samples designated for AAS testing.

(f) A trainer may request that a horse be placed on the veterinarian's list due to medically necessary treatment with AAS. The horse shall remain on the veterinarian's list:

1. (1) for 365 days;
2. (2) until the concentration of the drug or metabolite in urine or blood has fallen below the designated threshold for the administered AAS; or
3. (3) until the concentration of the drug or metabolite in urine or blood has fallen below the limit of detection for AAS that do not have a designated threshold, whichever is longer.

(Indiana Horse Racing Commission; 71 IAC 8-1-8; emergency rule filed Mar 12, 2008, 1:53 p.m.: 20080326-IR- 071080191ERA, eff Mar 11, 2008 [ IC 4-22-2-37.1 establishes the effectiveness of an emergency rule upon filing with the Publisher. LSA Document #08-191(E) was filed with the Publisher March 12, 2008.]; emergency rule filed May 12, 2008, 1:29 p.m.: 20080521-IR- 071080353ERA; readopted filed Nov 26, 2013, 11:25 a.m.: 20131225-IR-071130345RFA; emergency rule filed May 7, 2014, 2:27 p.m.: 20140514-IR-071140143ERA, eff May 15, 2014; readopted filed Oct 7, 2020, 2:27 p.m.: 20201104-IR- 071200406RFA; readopted filed Aug 3, 2023, 12:49 p.m.: 20230830-IR-071230428RFA)