Conn. Agencies Regs. § 22-332b-9
(a) The activity shall be under the direction of a person or persons with qualifications acceptable to the state department of health who shall be designated by the licensee as responsible for the operation of the facility licensed. Except for limited activities and with express approval of the commissioner of health, the following are the minimum acceptable qualifications;
(k) Surgery shall be performed under strict asepsis whenever the animal is expected to survive. No dog shall be used for two or more successive painful experiments unless it is a part of the original operation.
(l) When a surgical or experimental procedure has been performed on a dog which will continue to cause discomfort or pain to the animal or might significantly shorten its normal life expectancy, the animal shall be destroyed painlessly by intravenous injection of a drug acceptable to the commissioner of health. This shall be done promptly upon conclusion of a reasonable postoperative or post experimental observation period during which the animal shall be given care to minimize discomfort equivalent to that which would be rendered to human beings under similar circumstances.