15 C.F.R. § 971.603
(d) The monitoring of benthic impact shall involve the study of two types of areas, each selected by the permittee in consultation with NOAA, which areas shall be representative of the environmental characteristics of the permittee's site:
(2) An interim preservational reference area, located in a portion of a permit area tentatively determined: to be non-mineable, not to be scheduled for mining during the commercial recovery plan, or to be scheduled for mining late in the plan.
Reference areas may be selected provisionally prior to application for a commercial recovery permit.
(e) The following specific environmental parameters must be proposed for examination in the applicant's monitoring plan:
(1) Discharges—
(2) Upper water column—
(3) Lower water column and seafloor—