(a) Sample collection.

1. (1)

   1. (A) To collect a urine sample, the subject must first be instructed to void the bladder.
   2. (B) Approximately one-half (1/2) hour later (time not critical):

(i) The subject should again be requested to void the bladder; and

1. (ii) That specimen should be collected for analysis.
2. (2) Note. Urine is not to be collected as a postmortem sample.

(b) Sample size. Ten (10) to thirty (30) milliliters (cc) of urine shall be considered sufficient for analysis.

1. (c) Sample container.
2. (1) When urine collection is necessary, the specimen shall be deposited into a clean, dry, non-porous container and tightly capped or stoppered.
3. (2) Alcohol or other volatile organic solvents shall not be used to clean the container.

4. (3) The container shall be clearly identified with the following information:

   1. (A) Name of subject;
   2. (B) Date and time of first voiding and of the collection; and

   3. (C) Name or initials of person witnessing collection and sealing the sample.

      1. (d) Sample preservation.
5. (1) While not in transit or under examination, urine samples shall be refrigerated.
6. (2) If preservatives are used, a comment stating the type and amount should accompany the sample.

(e) Sample witness.

1. (1) The collection of the sample must be witnessed in order that its authenticity may be proven.
2. (2) It is to be properly marked for future identification and secured in a tamperproof manner.