(a)

1. (1) All bovine animals in approved herds are to be depopulated except steers and/or spayed heifers.
2. (2) Heifers over four hundred pounds (400 lbs.) body weight will be S-branded or spayed.

(b)

1. (1) All animals to be depopulated shall be branded and tagged within fifteen (15) days after approval of the depopulation.
2. (2) Within fifteen (15) days after the branding and tagging, the animals must be slaughtered at a federally-inspected slaughtering establishment.
3. (3) Any extensions of these time limits can be granted only by the federal veterinarian in charge.
4. (4) Thirty (30) days after the approval of the depopulation, there will be no sexually intact bovine animals on the premise.

(d)

1. (1) The premises are to be inspected by the Department of Agriculture personnel prior to restocking.
2. (2) If the inspector determines that certain areas or facilities need cleaning and disinfection, this must be accomplished prior to restocking.
3. (3) If the inspection reveal other species to be infected with Brucella abortus, said animals must be removed from the farm premises at least ninety (90) days prior to restocking.

(e) Restocking requirements.

1. (1) All female cattle must be official calfhood vaccinates.
2. (2) All test eligible cattle must have a negative brucellosis test within thirty (30) days prior to arrival at premises.

3. (3)

   1. (A) The cattle must be retested at the owner’s expense forty-five (45) to one hundred twenty (120) days after arrival.
   2. (B) However, additional tests may be required beyond one hundred twenty (120) days.